Prep HPLC Column, Bidentate C8, 4um, 100A. 21.2mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑P21‑150 Cogent Bidentate C8™ Preparative HPLC Column is a long‑format preparative column designed for purification workflows requiring higher resolution and improved fraction purity compared with shorter preparative formats. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under demanding preparative conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This non‑traditional chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it ideal for hydrophobic molecules, metabolites, and amine‑containing analytes when C18 retention is excessive.
With a 21.2 mm internal diameter and 150 mm length, this column delivers increased plate count and separation efficiency, enabling improved resolution of closely eluting compounds prior to fraction collection. This format is particularly valuable when higher purity fractions are required without moving to the longest (250 mm) configuration.
System Note: A specialized preparative HPLC system (sufficient high‑flow pump capacity, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size and length.
Why Choose the 21.2 × 150 mm Prep Bidentate C8™ Format
This configuration is commonly selected when:
C18 columns are too retentive for preparative targets
Higher resolution is needed beyond 21.2 × 100 mm formats
Large sample loads include closely related impurities
Improved fraction purity is required before collection
Balanced retention is needed for hydrophobic or basic analytes
The extended 150 mm length increases interaction time and separation efficiency, while TYPE‑C™ silica hydride chemistry ensures fast equilibration and long service life.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional preparative C8 columns
Very robust lifetime for repeated purification workflows
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Ideal alternative when C18 is overly retentive
Preparative Geometry with Enhanced Resolution
21.2 mm ID × 150 mm length
Higher plate count vs. shorter preparative columns
Supports tighter fraction cuts and improved purity
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for aggressive preparative methods
Typical Applications
Preparative purification and fraction collection
Hydrophobic molecule isolation
Metabolite purification
Amine‑containing analytes
Scale‑up from analytical or shorter preparative methods
Situations where C18 retention is excessive
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable